Detektion af NO ved anvendelse af guanylylcyklase og cGMP-fremstilling som et read-out-system
A method for measuring nitric oxide synthase activity, characterized in the method comprises; (a) providing a solution comprising a nitric oxide synthase and a suitable substrate under conditions which allow synthesis of nitric oxide; (b) adding guanylyl cyclase and GTP under conditions which allow...
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Zusammenfassung: | A method for measuring nitric oxide synthase activity, characterized in the method comprises; (a) providing a solution comprising a nitric oxide synthase and a suitable substrate under conditions which allow synthesis of nitric oxide; (b) adding guanylyl cyclase and GTP under conditions which allow synthesis of cGMP; (c) measuring cGMP. |
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