Verfahren zur Bestimmung der alpha-Amylase-Aktivität
A method for determining a-amylase activity which comprises bringing a sample into contact with an a-glucosidase in the presence of an a-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing term...
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| creator | EMI, SHIGENORI YAMAMOTO, YOSHIHIRO HATTORI, SHIZUO SOGABE, YUKIHIRO |
| description | A method for determining a-amylase activity which comprises bringing a sample into contact with an a-glucosidase in the presence of an a-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing terminal glucose is bonded to an optically measurable label at the 1-position by a-glucoside linkage or b-glucoside linkage, and whose non-reducing terminal glucose is modified by a substituent other than glucose, and the a-glucosidase being substantially capable of acting on glucose to which said label is bonded at the 1-position by a-glucoside linkage and on all maltooligosaccharides having 2 to 7 glucose units; and a reagent for determining a-amylase activity comprising said a-glucosidase and said a-amylase substrate. The present invention permits, in the determination of a-amylase activity, efficient auxiliary action of adjuvant enzyme, a determination with good sensitivity, and little reagent blank reaction. |
| format | Patent |
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The present invention permits, in the determination of a-amylase activity, efficient auxiliary action of adjuvant enzyme, a determination with good sensitivity, and little reagent blank reaction.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Verfahren zur Bestimmung der alpha-Amylase-Aktivität |
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