Zur Actinomyceten-DNS-Klonierung nützliches bifunktionelles Cosmid
The present invention relates to novel and useful cosmids for cloning and expressing the isolated DNA gene cluster which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids (cosmids) are isolated by screening a cosmi...
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| creator | RYAN, MICHAEL J., WEST MILFORD, NEW JERSEY 07483, US LOTVIN, JASON A., UNION, NEW JERSEY 07083, US STRATHY, NANCY, STATE OF NEW YORK 10952, US |
| description | The present invention relates to novel and useful cosmids for cloning and expressing the isolated DNA gene cluster which directs the synthesis of tetracycline and chlortetracycline, in a heterologous host such as Streptomyces lividans. The claimed plasmids (cosmids) are isolated by screening a cosmid library, constructed in Escherichia coli, for the expression of tetracycline resistance in S. lividans. Plasmids LP 127 and LP 128 are recovered from such tetracycline-resistant S. lividans isolates shown by HPLC analysis of agar and broth fermentations to direct the synthesis of tetracycline and chlortetracycline. |
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The claimed plasmids (cosmids) are isolated by screening a cosmid library, constructed in Escherichia coli, for the expression of tetracycline resistance in S. lividans. 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The claimed plasmids (cosmids) are isolated by screening a cosmid library, constructed in Escherichia coli, for the expression of tetracycline resistance in S. lividans. Plasmids LP 127 and LP 128 are recovered from such tetracycline-resistant S. lividans isolates shown by HPLC analysis of agar and broth fermentations to direct the synthesis of tetracycline and chlortetracycline.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Zur Actinomyceten-DNS-Klonierung nützliches bifunktionelles Cosmid |
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