Grossfermenter-Inokulation mit gefrorenen Zellen
An improved method of inoculating large scale fermenters with mammalian cells is provided. Instead of taking one or more ampules of frozen cells through a series of scale up steps in tissue culture flasks and/or roller bottles, the cells are inoculated into a small fermenter in advance of their anti...
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| creator | SCHWARTZ, BARRY D., REDWOOD CITY, CALIFORNIA 94061, US TRAWINSKI, JURGEN, RICHMOND, CALIFORNIA 94084, US |
| description | An improved method of inoculating large scale fermenters with mammalian cells is provided. Instead of taking one or more ampules of frozen cells through a series of scale up steps in tissue culture flasks and/or roller bottles, the cells are inoculated into a small fermenter in advance of their anticipated usage in fermenter inoculation. The cells are concentrated and dispensed into approximately 500 ml aliquots (or larger) and mixed with a cryopreservative solution. The aliquots are frozen in liquid nitrogen in specially designed freezer bags. The cells can thus be held indefinitely. When a fermenter run is to be initiated, a cell bag can simply be thawed and inoculated into the fermenter. Because the cells have been previously adapted to the fermenter medium, cell growth and production begin almost immediately after inoculation. |
| format | Patent |
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Instead of taking one or more ampules of frozen cells through a series of scale up steps in tissue culture flasks and/or roller bottles, the cells are inoculated into a small fermenter in advance of their anticipated usage in fermenter inoculation. The cells are concentrated and dispensed into approximately 500 ml aliquots (or larger) and mixed with a cryopreservative solution. The aliquots are frozen in liquid nitrogen in specially designed freezer bags. The cells can thus be held indefinitely. When a fermenter run is to be initiated, a cell bag can simply be thawed and inoculated into the fermenter. Because the cells have been previously adapted to the fermenter medium, cell growth and production begin almost immediately after inoculation.</description><edition>5</edition><language>ger</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PEST REPELLANTS OR ATTRACTANTS ; PLANT GROWTH REGULATORS ; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>1994</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19941208&DB=EPODOC&CC=DE&NR=69011177T2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19941208&DB=EPODOC&CC=DE&NR=69011177T2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SCHWARTZ, BARRY D., REDWOOD CITY, CALIFORNIA 94061, US</creatorcontrib><creatorcontrib>TRAWINSKI, JURGEN, RICHMOND, CALIFORNIA 94084, US</creatorcontrib><title>Grossfermenter-Inokulation mit gefrorenen Zellen</title><description>An improved method of inoculating large scale fermenters with mammalian cells is provided. 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Instead of taking one or more ampules of frozen cells through a series of scale up steps in tissue culture flasks and/or roller bottles, the cells are inoculated into a small fermenter in advance of their anticipated usage in fermenter inoculation. The cells are concentrated and dispensed into approximately 500 ml aliquots (or larger) and mixed with a cryopreservative solution. The aliquots are frozen in liquid nitrogen in specially designed freezer bags. The cells can thus be held indefinitely. When a fermenter run is to be initiated, a cell bag can simply be thawed and inoculated into the fermenter. Because the cells have been previously adapted to the fermenter medium, cell growth and production begin almost immediately after inoculation.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Grossfermenter-Inokulation mit gefrorenen Zellen |
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