Fluoreszenzabbildung mittels Telezentrizität

Fluoreszenzabbildung mittels Telezentrizität

The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to...

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Hauptverfasser: KOENIG, JOACHIM, GUSE, FRANK, HESSLING, MARTIN, HECKMANN, HANS-GEORG, GUTEKUNST, MARTIN
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator KOENIG, JOACHIM
GUSE, FRANK
HESSLING, MARTIN
HECKMANN, HANS-GEORG
GUTEKUNST, MARTIN
description The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image multiplex real time PCR or to read out DNA microarrays. According to a preferred embodiment the device comprises holding means (1) for holding a planar support (2) with an assembly of multiple individual sites (3); at least one light source (4) for emitting light comprising at least one excitation frequency; a transducer (5) for receiving fluorescence signals from said sites; a field lens (6); an excitation lens arrangement (10), and an imaging lens arrangement (11), wherein said excitation light path and said fluorescence imaging light path are telecentric on the object side of said field lens and wherein the angle of incidence of the excitation light with said planar support is larger than 0°.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Fluoreszenzabbildung mittels Telezentrizität
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