METHODE ZUM NACHWEIS VON MINDERHEITSGENOMEN IN VIRALEN QUASI-SPEZIES UNTER VERWENDUNG VON DNA-MIKROCHIPS
This method permits minority genomes to be detected, more specifically, minority memory genomes of a nucleic acid population present in less than 50% of a viral quasispecies and that contain at least one mutation in comparison to the majority genome of the quasispecies and consists in: extraction of...
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| creator | ARIAS ESTEBAN, ARMANDO GOMEZ CASTILLA BARANOWSKI, ERIC MARTIN RUIZ-JARABO, CARMEN PARRO GARCIA DOMINGO SOLANS, ESTEBAN BRIONES LLORENTE ESCARMIS HOMS, CRISTINA |
| description | This method permits minority genomes to be detected, more specifically, minority memory genomes of a nucleic acid population present in less than 50% of a viral quasispecies and that contain at least one mutation in comparison to the majority genome of the quasispecies and consists in: extraction of the nucleic acid from a sample suspected to contain this viral quasispecies; b) amplification of at least one nucleic acid fragment from this quasispecies; and c) detection and analysis of the existence of minority genomes using techniques based on the use of DNA microchips, Heteroduplex trace assay (HTA) and molecular cloning. The method can be used in genetic diagnostics of viral diseases. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHODE ZUM NACHWEIS VON MINDERHEITSGENOMEN IN VIRALEN QUASI-SPEZIES UNTER VERWENDUNG VON DNA-MIKROCHIPS |
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