Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining

Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining

Investigation of cells from body fluids, by detection of cell-associated determinants, comprises: (a) applying the sample to a carrier for microscopy using filtration; (b) carrying out antigen-antibody reaction by adding a monoclonal antibody against the cell-associated antigen; (c) removing non-bon...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: GERKE, ROGER, 59269 BECKUM, DE, SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE, EBERT, THOMAS., 4040 NEUSS, DE, DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE
Format: Patent
Sprache:eng ; ger
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator GERKE, ROGER, 59269 BECKUM, DE
SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE
EBERT, THOMAS., 4040 NEUSS, DE
DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE
description Investigation of cells from body fluids, by detection of cell-associated determinants, comprises: (a) applying the sample to a carrier for microscopy using filtration; (b) carrying out antigen-antibody reaction by adding a monoclonal antibody against the cell-associated antigen; (c) removing non-bonded antibody; (d) carrying out a staining reaction; and (e) identifying antigen-positive and antigen negative cells by counter-staining. The sample is pre-fixed by treatment with a soln. which lyses the erythrocytes (pref. with acetic acid) and preserves the non-lysed cells (pref. with methanol), esp. a soln. contg. 10 pts. glacial acetic acid, 45 pts. methanol and 45 pts. water. USE/ADVANTAGE - The method is esp. used for diagnosis of urothelial (bladder) tumours, by testing of urine. Typical of many other exemplified applications are haemotological tests for tumour or parasitic diseases, testing vaginal swabs for papilloma virus and genetic tests on amniotic fluid. Some tests involve nucleic acid hybridisation rather than antigen-antibody reaction. The process is rapid, taking a total of 30 min., compared with 2.5-5 hrs. for prior art methods. Use of cytocentrifuges (with associated time, cost and cell loss) is avoided.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_DE4237649A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>DE4237649A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_DE4237649A13</originalsourceid><addsrcrecordid>eNqNjjFOQzEQRH9DgQJ3mAP8FJAIRIkgETWij_bb66-VNl7L9i98Gq7KInEAqpFm3ozmdvr-pCIRYXRTWyWQgjLpaNJgCYvFgaSbxBm8YquSGXtwK0hWsSjFyBWBcnCJQms2r85YBqgUHZJXBFZt6OZYreKch0m0V-pieYZcr5vPVqbwa_iBiNZJspfvpptE2vj-T3cTzqevt489F7v4DQqcuV_eT8fHw_PT8eX14fAP5Adn6FNv</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining</title><source>esp@cenet</source><creator>GERKE, ROGER, 59269 BECKUM, DE ; SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE ; EBERT, THOMAS., 4040 NEUSS, DE ; DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</creator><creatorcontrib>GERKE, ROGER, 59269 BECKUM, DE ; SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE ; EBERT, THOMAS., 4040 NEUSS, DE ; DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</creatorcontrib><description>Investigation of cells from body fluids, by detection of cell-associated determinants, comprises: (a) applying the sample to a carrier for microscopy using filtration; (b) carrying out antigen-antibody reaction by adding a monoclonal antibody against the cell-associated antigen; (c) removing non-bonded antibody; (d) carrying out a staining reaction; and (e) identifying antigen-positive and antigen negative cells by counter-staining. The sample is pre-fixed by treatment with a soln. which lyses the erythrocytes (pref. with acetic acid) and preserves the non-lysed cells (pref. with methanol), esp. a soln. contg. 10 pts. glacial acetic acid, 45 pts. methanol and 45 pts. water. USE/ADVANTAGE - The method is esp. used for diagnosis of urothelial (bladder) tumours, by testing of urine. Typical of many other exemplified applications are haemotological tests for tumour or parasitic diseases, testing vaginal swabs for papilloma virus and genetic tests on amniotic fluid. Some tests involve nucleic acid hybridisation rather than antigen-antibody reaction. The process is rapid, taking a total of 30 min., compared with 2.5-5 hrs. for prior art methods. Use of cytocentrifuges (with associated time, cost and cell loss) is avoided.</description><edition>5</edition><language>eng ; ger</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>1994</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19940511&amp;DB=EPODOC&amp;CC=DE&amp;NR=4237649A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=19940511&amp;DB=EPODOC&amp;CC=DE&amp;NR=4237649A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GERKE, ROGER, 59269 BECKUM, DE</creatorcontrib><creatorcontrib>SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE</creatorcontrib><creatorcontrib>EBERT, THOMAS., 4040 NEUSS, DE</creatorcontrib><creatorcontrib>DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</creatorcontrib><title>Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining</title><description>Investigation of cells from body fluids, by detection of cell-associated determinants, comprises: (a) applying the sample to a carrier for microscopy using filtration; (b) carrying out antigen-antibody reaction by adding a monoclonal antibody against the cell-associated antigen; (c) removing non-bonded antibody; (d) carrying out a staining reaction; and (e) identifying antigen-positive and antigen negative cells by counter-staining. The sample is pre-fixed by treatment with a soln. which lyses the erythrocytes (pref. with acetic acid) and preserves the non-lysed cells (pref. with methanol), esp. a soln. contg. 10 pts. glacial acetic acid, 45 pts. methanol and 45 pts. water. USE/ADVANTAGE - The method is esp. used for diagnosis of urothelial (bladder) tumours, by testing of urine. Typical of many other exemplified applications are haemotological tests for tumour or parasitic diseases, testing vaginal swabs for papilloma virus and genetic tests on amniotic fluid. Some tests involve nucleic acid hybridisation rather than antigen-antibody reaction. The process is rapid, taking a total of 30 min., compared with 2.5-5 hrs. for prior art methods. Use of cytocentrifuges (with associated time, cost and cell loss) is avoided.</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1994</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjFOQzEQRH9DgQJ3mAP8FJAIRIkgETWij_bb66-VNl7L9i98Gq7KInEAqpFm3ozmdvr-pCIRYXRTWyWQgjLpaNJgCYvFgaSbxBm8YquSGXtwK0hWsSjFyBWBcnCJQms2r85YBqgUHZJXBFZt6OZYreKch0m0V-pieYZcr5vPVqbwa_iBiNZJspfvpptE2vj-T3cTzqevt489F7v4DQqcuV_eT8fHw_PT8eX14fAP5Adn6FNv</recordid><startdate>19940511</startdate><enddate>19940511</enddate><creator>GERKE, ROGER, 59269 BECKUM, DE</creator><creator>SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE</creator><creator>EBERT, THOMAS., 4040 NEUSS, DE</creator><creator>DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</creator><scope>EVB</scope></search><sort><creationdate>19940511</creationdate><title>Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining</title><author>GERKE, ROGER, 59269 BECKUM, DE ; SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE ; EBERT, THOMAS., 4040 NEUSS, DE ; DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_DE4237649A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; ger</language><creationdate>1994</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>GERKE, ROGER, 59269 BECKUM, DE</creatorcontrib><creatorcontrib>SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE</creatorcontrib><creatorcontrib>EBERT, THOMAS., 4040 NEUSS, DE</creatorcontrib><creatorcontrib>DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GERKE, ROGER, 59269 BECKUM, DE</au><au>SCHMITZ-DRAEGER, BERND., 4040 NEUSS, DE</au><au>EBERT, THOMAS., 4040 NEUSS, DE</au><au>DECKEN, KLAUS-BIOL., 4000 DUESSELDORF, DE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining</title><date>1994-05-11</date><risdate>1994</risdate><abstract>Investigation of cells from body fluids, by detection of cell-associated determinants, comprises: (a) applying the sample to a carrier for microscopy using filtration; (b) carrying out antigen-antibody reaction by adding a monoclonal antibody against the cell-associated antigen; (c) removing non-bonded antibody; (d) carrying out a staining reaction; and (e) identifying antigen-positive and antigen negative cells by counter-staining. The sample is pre-fixed by treatment with a soln. which lyses the erythrocytes (pref. with acetic acid) and preserves the non-lysed cells (pref. with methanol), esp. a soln. contg. 10 pts. glacial acetic acid, 45 pts. methanol and 45 pts. water. USE/ADVANTAGE - The method is esp. used for diagnosis of urothelial (bladder) tumours, by testing of urine. Typical of many other exemplified applications are haemotological tests for tumour or parasitic diseases, testing vaginal swabs for papilloma virus and genetic tests on amniotic fluid. Some tests involve nucleic acid hybridisation rather than antigen-antibody reaction. The process is rapid, taking a total of 30 min., compared with 2.5-5 hrs. for prior art methods. Use of cytocentrifuges (with associated time, cost and cell loss) is avoided.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng ; ger
recordid cdi_epo_espacenet_DE4237649A1
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Rapid cytological analysis of body fluid, eg urine - esp for bladder cancer diagnosis, by applying cells to carrier by filtration, immune reaction and staining
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T15%3A58%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=GERKE,%20ROGER,%2059269%20BECKUM,%20DE&rft.date=1994-05-11&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EDE4237649A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true