STREPTOMYCETES-PLASMIDE MIT SEHR HOHER KOPIENZAHL
The present invention discloses an ∼1.4 kb BclI-Sau3A restriction fragment containing the minimal replicon of the Streptomycescoelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicati...
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creator | LARSON, JEFFREY LYNN, INDIANAPOLIS INDIANA 46260, US REYNOLDS, PATRICIA ANN, INDIANAPOLIS INDIANA 46254, US HERSHBERGER, CHARLES LEE, NEW PALESTINE INDIANA 46163, US |
description | The present invention discloses an ∼1.4 kb BclI-Sau3A restriction fragment containing the minimal replicon of the Streptomycescoelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes. |
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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. 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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. 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This minimal replicon was deduced from plasmid derivatives constructed by invitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyceslividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | STREPTOMYCETES-PLASMIDE MIT SEHR HOHER KOPIENZAHL |
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