DE2920764
Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to...
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| creator | COMER, MICHAEL JAMES, 8120 WEILHEIM, DE ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB |
| description | Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40 DEG -65 DEG C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000+/-30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30 DEG C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids. The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer. |
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Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40 DEG -65 DEG C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000+/-30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30 DEG C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids. The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.</description><edition>4</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1988</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19880204&DB=EPODOC&CC=DE&NR=2920764C2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19880204&DB=EPODOC&CC=DE&NR=2920764C2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>COMER, MICHAEL JAMES, 8120 WEILHEIM, DE</creatorcontrib><creatorcontrib>ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB</creatorcontrib><creatorcontrib>SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB</creatorcontrib><creatorcontrib>BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB</creatorcontrib><title>DE2920764</title><description>Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40 DEG -65 DEG C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000+/-30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30 DEG C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids. The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1988</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZOB0cTWyNDIwNzPhYWBNS8wpTuWF0twMCm6uIc4euqkF-fGpxQWJyal5qSXxcPXORsZEKAEAzjoaeQ</recordid><startdate>19880204</startdate><enddate>19880204</enddate><creator>COMER, MICHAEL JAMES, 8120 WEILHEIM, DE</creator><creator>ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB</creator><creator>SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB</creator><creator>BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB</creator><scope>EVB</scope></search><sort><creationdate>19880204</creationdate><title>DE2920764</title><author>COMER, MICHAEL JAMES, 8120 WEILHEIM, DE ; ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB ; SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB ; BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_DE2920764C23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1988</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>COMER, MICHAEL JAMES, 8120 WEILHEIM, DE</creatorcontrib><creatorcontrib>ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB</creatorcontrib><creatorcontrib>SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB</creatorcontrib><creatorcontrib>BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>COMER, MICHAEL JAMES, 8120 WEILHEIM, DE</au><au>ATKINSON, ANTHONY, SALISBURY, WILTSHIRE, GB</au><au>SHARP, RICHARD JAMES, SALISBURY, WILTSHIRE, GB</au><au>BRUTON, CHRISTOPHER JOHN, KEW, SURREY, GB</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>DE2920764</title><date>1988-02-04</date><risdate>1988</risdate><abstract>Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable strains are described. The strains are grown in conventional culture media, preferably containing 0.05 to 4.0%, especially 0.1 to 1.0%, by weight of glycerol or a glycerol analogue at 40 DEG -65 DEG C. and pH 5 to 8. The enzyme is isolated by conventional cell disruption and separation techniques, and typically has a molcular weight of 240,000+/-30,000, composed of four similar sub-units, and a specific activity of greater than 5 Units per mg protein at 30 DEG C. by the modified assay described. They may be stored as aqueous solutions or a freeze dried solids. The enzymes may be used for assay of serum triglycerides by conventional assay methods, but preferably by the nictotinamide adenine dinucleotide spectrophotometric assay at a pH of 7 to 8.8. The pH is preferably controlled by an amine, especially triethanolamine/HCl, buffer.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | DE2920764 |
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