Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay
The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Patent |
| Sprache: | ger |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | KOPINKE, HOLM., 04357 LEIPZIG, DE GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE RENNEBERG, ILKA., 13187 BERLIN, DE STREHLITZ, BEATE., 04129 LEIPZIG, DE |
| description | The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication of affinity reactions and is particularly suitable in the form of a one-step affinity sensor for in situ analytics. The invention is also directed to the use of phenol oxidase as a marker enzyme for the affine binding partners in an electrochemical affinity sensor or assay, and to the use of an enzyme hydrolyzing phenolic compounds as marker enzyme for the affine binding partners, in combination with a phenol oxidase as catalyst for the amplifying reaction in an electrochemical affinity assay. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_DE19622458C2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>DE19622458C2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_DE19622458C23</originalsourceid><addsrcrecordid>eNqNjD0OgkAQhWksjHqH9QAU4k-0RMTY2NmTDQ4wEQbcGTBwCK9gw024mGtiYWn1vffy8o2dZ0hdW2hBjjMXcriJKeMMik8Ho0IkGwyKuH6SIKEMvTADcWlUVxt1rzUJihU0QGoPLFgUNaWqKUn5pPNW7I6kHkM_vAymtp3hihY1XdWPVDPrduqMEp0zzL6cOPNjeAlOLlRlBFzpGAgkOoSL3cbzVutt4C3_-bwBoOxTJA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay</title><source>esp@cenet</source><creator>KOPINKE, HOLM., 04357 LEIPZIG, DE ; GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE ; RENNEBERG, ILKA., 13187 BERLIN, DE ; STREHLITZ, BEATE., 04129 LEIPZIG, DE</creator><creatorcontrib>KOPINKE, HOLM., 04357 LEIPZIG, DE ; GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE ; RENNEBERG, ILKA., 13187 BERLIN, DE ; STREHLITZ, BEATE., 04129 LEIPZIG, DE</creatorcontrib><description>The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication of affinity reactions and is particularly suitable in the form of a one-step affinity sensor for in situ analytics. The invention is also directed to the use of phenol oxidase as a marker enzyme for the affine binding partners in an electrochemical affinity sensor or assay, and to the use of an enzyme hydrolyzing phenolic compounds as marker enzyme for the affine binding partners, in combination with a phenol oxidase as catalyst for the amplifying reaction in an electrochemical affinity assay.</description><edition>6</edition><language>ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1998</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980326&DB=EPODOC&CC=DE&NR=19622458C2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980326&DB=EPODOC&CC=DE&NR=19622458C2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KOPINKE, HOLM., 04357 LEIPZIG, DE</creatorcontrib><creatorcontrib>GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE</creatorcontrib><creatorcontrib>RENNEBERG, ILKA., 13187 BERLIN, DE</creatorcontrib><creatorcontrib>STREHLITZ, BEATE., 04129 LEIPZIG, DE</creatorcontrib><title>Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay</title><description>The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication of affinity reactions and is particularly suitable in the form of a one-step affinity sensor for in situ analytics. The invention is also directed to the use of phenol oxidase as a marker enzyme for the affine binding partners in an electrochemical affinity sensor or assay, and to the use of an enzyme hydrolyzing phenolic compounds as marker enzyme for the affine binding partners, in combination with a phenol oxidase as catalyst for the amplifying reaction in an electrochemical affinity assay.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1998</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjD0OgkAQhWksjHqH9QAU4k-0RMTY2NmTDQ4wEQbcGTBwCK9gw024mGtiYWn1vffy8o2dZ0hdW2hBjjMXcriJKeMMik8Ho0IkGwyKuH6SIKEMvTADcWlUVxt1rzUJihU0QGoPLFgUNaWqKUn5pPNW7I6kHkM_vAymtp3hihY1XdWPVDPrduqMEp0zzL6cOPNjeAlOLlRlBFzpGAgkOoSL3cbzVutt4C3_-bwBoOxTJA</recordid><startdate>19980326</startdate><enddate>19980326</enddate><creator>KOPINKE, HOLM., 04357 LEIPZIG, DE</creator><creator>GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE</creator><creator>RENNEBERG, ILKA., 13187 BERLIN, DE</creator><creator>STREHLITZ, BEATE., 04129 LEIPZIG, DE</creator><scope>EVB</scope></search><sort><creationdate>19980326</creationdate><title>Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay</title><author>KOPINKE, HOLM., 04357 LEIPZIG, DE ; GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE ; RENNEBERG, ILKA., 13187 BERLIN, DE ; STREHLITZ, BEATE., 04129 LEIPZIG, DE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_DE19622458C23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>ger</language><creationdate>1998</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KOPINKE, HOLM., 04357 LEIPZIG, DE</creatorcontrib><creatorcontrib>GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE</creatorcontrib><creatorcontrib>RENNEBERG, ILKA., 13187 BERLIN, DE</creatorcontrib><creatorcontrib>STREHLITZ, BEATE., 04129 LEIPZIG, DE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KOPINKE, HOLM., 04357 LEIPZIG, DE</au><au>GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE</au><au>RENNEBERG, ILKA., 13187 BERLIN, DE</au><au>STREHLITZ, BEATE., 04129 LEIPZIG, DE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay</title><date>1998-03-26</date><risdate>1998</risdate><abstract>The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication of affinity reactions and is particularly suitable in the form of a one-step affinity sensor for in situ analytics. The invention is also directed to the use of phenol oxidase as a marker enzyme for the affine binding partners in an electrochemical affinity sensor or assay, and to the use of an enzyme hydrolyzing phenolic compounds as marker enzyme for the affine binding partners, in combination with a phenol oxidase as catalyst for the amplifying reaction in an electrochemical affinity assay.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | ger |
| recordid | cdi_epo_espacenet_DE19622458C2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T16%3A30%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=KOPINKE,%20HOLM.,%2004357%20LEIPZIG,%20DE&rft.date=1998-03-26&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EDE19622458C2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |