THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING
PCT No. PCT/EP95/00926 Sec. 371 Date Sep. 13, 1996 Sec. 102(e) Date Sep. 13, 1996 PCT Filed Mar. 13, 1995 PCT Pub. No. WO95/25168 PCT Pub. Date Sep. 21, 1995Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | STERN ANNE STERN ANNE FISCHER STEPHAN FISCHER STEPHAN KOHNERT ULRICH KOHNERT ULRICH |
| description | PCT No. PCT/EP95/00926 Sec. 371 Date Sep. 13, 1996 Sec. 102(e) Date Sep. 13, 1996 PCT Filed Mar. 13, 1995 PCT Pub. No. WO95/25168 PCT Pub. Date Sep. 21, 1995Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of an inhibitor DE-3 from Erythrina caffra, removing unbound components from the protein mixture, detaching the serine protease from the inhibitor and separating the immobilized inhibitor from the soluble serine protease and isolating serine protease which is characterized in that a polypeptide is used as the polypeptide which is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid. This inhibitor is distinguished by an improved specific activity and is particularly suitable for the purification of plasminogen activators such as tissue plasminogen activators (t-PA and derivatives). |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CZ252996A3</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CZ252996A3</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CZ252996A33</originalsourceid><addsrcrecordid>eNrjZOgI8XBVCA12VfB3Uwhydfb3dfL0c_QLUfD08_B08gzxD1JwC_L3VXAN8gzxCAJKKTgrkKzD0c0tyFHBDSjj7OPq6Ofp566AKcTDwJqWmFOcyguluRnk3VxDnD10Uwvy41OLCxKTU_NSS-Kdo4xMjSwtzRyNjQmrAAANXD0P</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING</title><source>esp@cenet</source><creator>STERN ANNE STERN ANNE ; FISCHER STEPHAN FISCHER STEPHAN ; KOHNERT ULRICH KOHNERT ULRICH</creator><creatorcontrib>STERN ANNE STERN ANNE ; FISCHER STEPHAN FISCHER STEPHAN ; KOHNERT ULRICH KOHNERT ULRICH</creatorcontrib><description>PCT No. PCT/EP95/00926 Sec. 371 Date Sep. 13, 1996 Sec. 102(e) Date Sep. 13, 1996 PCT Filed Mar. 13, 1995 PCT Pub. No. WO95/25168 PCT Pub. Date Sep. 21, 1995Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of an inhibitor DE-3 from Erythrina caffra, removing unbound components from the protein mixture, detaching the serine protease from the inhibitor and separating the immobilized inhibitor from the soluble serine protease and isolating serine protease which is characterized in that a polypeptide is used as the polypeptide which is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid. This inhibitor is distinguished by an improved specific activity and is particularly suitable for the purification of plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1996</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19961113&DB=EPODOC&CC=CZ&NR=252996A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19961113&DB=EPODOC&CC=CZ&NR=252996A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>STERN ANNE STERN ANNE</creatorcontrib><creatorcontrib>FISCHER STEPHAN FISCHER STEPHAN</creatorcontrib><creatorcontrib>KOHNERT ULRICH KOHNERT ULRICH</creatorcontrib><title>THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING</title><description>PCT No. PCT/EP95/00926 Sec. 371 Date Sep. 13, 1996 Sec. 102(e) Date Sep. 13, 1996 PCT Filed Mar. 13, 1995 PCT Pub. No. WO95/25168 PCT Pub. Date Sep. 21, 1995Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of an inhibitor DE-3 from Erythrina caffra, removing unbound components from the protein mixture, detaching the serine protease from the inhibitor and separating the immobilized inhibitor from the soluble serine protease and isolating serine protease which is characterized in that a polypeptide is used as the polypeptide which is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid. This inhibitor is distinguished by an improved specific activity and is particularly suitable for the purification of plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1996</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZOgI8XBVCA12VfB3Uwhydfb3dfL0c_QLUfD08_B08gzxD1JwC_L3VXAN8gzxCAJKKTgrkKzD0c0tyFHBDSjj7OPq6Ofp566AKcTDwJqWmFOcyguluRnk3VxDnD10Uwvy41OLCxKTU_NSS-Kdo4xMjSwtzRyNjQmrAAANXD0P</recordid><startdate>19961113</startdate><enddate>19961113</enddate><creator>STERN ANNE STERN ANNE</creator><creator>FISCHER STEPHAN FISCHER STEPHAN</creator><creator>KOHNERT ULRICH KOHNERT ULRICH</creator><scope>EVB</scope></search><sort><creationdate>19961113</creationdate><title>THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING</title><author>STERN ANNE STERN ANNE ; FISCHER STEPHAN FISCHER STEPHAN ; KOHNERT ULRICH KOHNERT ULRICH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CZ252996A33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1996</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>STERN ANNE STERN ANNE</creatorcontrib><creatorcontrib>FISCHER STEPHAN FISCHER STEPHAN</creatorcontrib><creatorcontrib>KOHNERT ULRICH KOHNERT ULRICH</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>STERN ANNE STERN ANNE</au><au>FISCHER STEPHAN FISCHER STEPHAN</au><au>KOHNERT ULRICH KOHNERT ULRICH</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING</title><date>1996-11-13</date><risdate>1996</risdate><abstract>PCT No. PCT/EP95/00926 Sec. 371 Date Sep. 13, 1996 Sec. 102(e) Date Sep. 13, 1996 PCT Filed Mar. 13, 1995 PCT Pub. No. WO95/25168 PCT Pub. Date Sep. 21, 1995Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of an inhibitor DE-3 from Erythrina caffra, removing unbound components from the protein mixture, detaching the serine protease from the inhibitor and separating the immobilized inhibitor from the soluble serine protease and isolating serine protease which is characterized in that a polypeptide is used as the polypeptide which is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid. This inhibitor is distinguished by an improved specific activity and is particularly suitable for the purification of plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_CZ252996A3 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA C THE USE OF RECOMBINANT INHIBITOR FROM ERITHRINA CAFFRA FOR CLEANING AFFRA FOR CLEANING |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T01%3A46%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=STERN%20ANNE%20STERN%20ANNE&rft.date=1996-11-13&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECZ252996A3%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |