Micro-fluidic chip for single-cell isolated culture of insect symbiotic fungi

The utility model discloses a micro-fluidic chip for single-cell isolated culture of insect symbiotic fungi, and relates to the technical field of micro-fluidic chips, the micro-fluidic chip is divided into three layers of structures from top to bottom, and the three layers of structures are a first PDMS plate, a second PDMS film and a third PDMS plate in sequence; the first layer of PDMS plate is provided with a total sample inlet, a total sample outlet, a micro-chamber sample outlet and a culture solution channel loaded with micro-chambers, and each micro-chamber comprises a straight micro-chamber main channel, a semicircular micro-chamber side channel and a micro-chamber sample outlet channel; the third layer of PDMS plate is provided with an outer air valve group for connecting and disconnecting a sample outlet channel of the micro-cavity and an inner air valve group for connecting and disconnecting a side channel of the micro-cavity; the utility model provides the micro-fluidic chip capable of being used