Conditional touchdown multiplex polymerase chain reaction
A high-throughput and cost-effective method for simultaneous amplification of target DNA sequences with high fidelity and workable rate is achieved by a two-stage amplification incorporating multiplex PCR with conditional touchdown strategies. This improved multiplex PCR comprises a simultaneous PCR...
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| creator | YUCHI,CHENG HWANG |
| description | A high-throughput and cost-effective method for simultaneous amplification of target DNA sequences with high fidelity and workable rate is achieved by a two-stage amplification incorporating multiplex PCR with conditional touchdown strategies. This improved multiplex PCR comprises a simultaneous PCR and a specific PCR, and either one or both of the amplification steps are performed with a touchdown strategy, of which loose touchdown strategy is applied with a temperature lower than the optimized annealing temperature, and stringent touchdown strategy is applied with a temperature higher than the optimized annealing temperature. |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Conditional touchdown multiplex polymerase chain reaction |
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