Color reagent, concentration measuring kit, method of concentration measuring and sensor chip for use therein
A method of concentration measuring in which the concentration of measuring subject substance in minute amounts of analyte can be measured in a short period of time and in which even when the amount of analyte measured is inaccurate, the concentration of measuring subject substance can be accurately...
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| creator | UCHIYAMA KENICHI,OOMIYA KAYOKO,KISHIMOTO ISAO,HIRATA MASAMI,ETO HIDEO,TONO ICHIRO,UEMATSU IKUO,KASAI SHINGO,TAKASE TOMOHIRO,HONJOH TSUTOMU,SUGITANI MASANORI |
| description | A method of concentration measuring in which the concentration of measuring subject substance in minute amounts of analyte can be measured in a short period of time and in which even when the amount of analyte measured is inaccurate, the concentration of measuring subject substance can be accurately measured. There is provided a method of measuring the concentration of measuring subject substance with the use of a sensor chip having a light guide layer and, disposed on the surface thereof, an antibody immobilization layer, which method comprises dropping an analyte solution and an enzyme-labeled antibody solution onto the antibody immobilization layer of the sensor chip to thereby carry out an antigen antibody reaction so as to fix the analyte; subsequently dropping a color reagent solution to thereby form coloring and precipitating enzymic reaction products so that the enzymic reaction products are deposited in the antibody immobilization layer; causing light incident on the sensor chip from outside to be wholly reflected by the antibody immobilization layer; and observing any change of physical quantity of the wholly reflected light. |
| format | Patent |
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There is provided a method of measuring the concentration of measuring subject substance with the use of a sensor chip having a light guide layer and, disposed on the surface thereof, an antibody immobilization layer, which method comprises dropping an analyte solution and an enzyme-labeled antibody solution onto the antibody immobilization layer of the sensor chip to thereby carry out an antigen antibody reaction so as to fix the analyte; subsequently dropping a color reagent solution to thereby form coloring and precipitating enzymic reaction products so that the enzymic reaction products are deposited in the antibody immobilization layer; causing light incident on the sensor chip from outside to be wholly reflected by the antibody immobilization layer; and observing any change of physical quantity of the wholly reflected light.</description><language>eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20061004&DB=EPODOC&CC=CN&NR=1842708A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20061004&DB=EPODOC&CC=CN&NR=1842708A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>UCHIYAMA KENICHI,OOMIYA KAYOKO,KISHIMOTO ISAO,HIRATA MASAMI,ETO HIDEO,TONO ICHIRO,UEMATSU IKUO,KASAI SHINGO,TAKASE TOMOHIRO,HONJOH TSUTOMU,SUGITANI MASANORI</creatorcontrib><title>Color reagent, concentration measuring kit, method of concentration measuring and sensor chip for use therein</title><description>A method of concentration measuring in which the concentration of measuring subject substance in minute amounts of analyte can be measured in a short period of time and in which even when the amount of analyte measured is inaccurate, the concentration of measuring subject substance can be accurately measured. 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There is provided a method of measuring the concentration of measuring subject substance with the use of a sensor chip having a light guide layer and, disposed on the surface thereof, an antibody immobilization layer, which method comprises dropping an analyte solution and an enzyme-labeled antibody solution onto the antibody immobilization layer of the sensor chip to thereby carry out an antigen antibody reaction so as to fix the analyte; subsequently dropping a color reagent solution to thereby form coloring and precipitating enzymic reaction products so that the enzymic reaction products are deposited in the antibody immobilization layer; causing light incident on the sensor chip from outside to be wholly reflected by the antibody immobilization layer; and observing any change of physical quantity of the wholly reflected light.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS SPIRITS TESTING VINEGAR WINE |
| title | Color reagent, concentration measuring kit, method of concentration measuring and sensor chip for use therein |
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