Process for preparing highly pure albumin solution
A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatogr...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON |
| description | A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN1810834A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN1810834A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN1810834A3</originalsourceid><addsrcrecordid>eNrjZDAKKMpPTi0uVkjLL1IoKEotSCzKzEtXyMhMz8ipVCgoLUpVSMxJKs3NzFMozs8pLcnMz-NhYE1LzClO5YXS3Azybq4hzh66qQX58anFBYnJqXmpJfHOfoYWhgYWxiaOxoRVAABFEiub</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Process for preparing highly pure albumin solution</title><source>esp@cenet</source><creator>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</creator><creatorcontrib>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</creatorcontrib><description>A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060802&DB=EPODOC&CC=CN&NR=1810834A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060802&DB=EPODOC&CC=CN&NR=1810834A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</creatorcontrib><title>Process for preparing highly pure albumin solution</title><description>A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2006</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDAKKMpPTi0uVkjLL1IoKEotSCzKzEtXyMhMz8ipVCgoLUpVSMxJKs3NzFMozs8pLcnMz-NhYE1LzClO5YXS3Azybq4hzh66qQX58anFBYnJqXmpJfHOfoYWhgYWxiaOxoRVAABFEiub</recordid><startdate>20060802</startdate><enddate>20060802</enddate><creator>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</creator><scope>EVB</scope></search><sort><creationdate>20060802</creationdate><title>Process for preparing highly pure albumin solution</title><author>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN1810834A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2006</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VAN URK HENDRIK,MEAD DAVID JOHN,MORTON PHILIP HARVEY,CARTWRIGHT ANDREW JOHN,CAMERON JASON,BALLANCE DAVID JAMES,GRANDGEORGE MICHEL GASTON JOSE,BEREZENKO STEPHEN,WOODROW JOHN RODNEY,SLEEP DARRELL,VERON</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for preparing highly pure albumin solution</title><date>2006-08-02</date><risdate>2006</risdate><abstract>A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_CN1810834A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Process for preparing highly pure albumin solution |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T17%3A33%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=VAN%20URK%20HENDRIK,MEAD%20DAVID%20JOHN,MORTON%20PHILIP%20HARVEY,CARTWRIGHT%20ANDREW%20JOHN,CAMERON%20JASON,BALLANCE%20DAVID%20JAMES,GRANDGEORGE%20MICHEL%20GASTON%20JOSE,BEREZENKO%20STEPHEN,WOODROW%20JOHN%20RODNEY,SLEEP%20DARRELL,VERON&rft.date=2006-08-02&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN1810834A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |