Method for cloning in the rat by nuclear transfer

Provided is a method for production of reconstituted rat embryos by nuclear transfer including the following steps: (a) removal of a rat ovocyte and maintenance in an appropriate medium; b) insertion of a rat cell nucleus into the recipient rat ovocyte previously maintained in vitro to obtain a reco...

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1. Verfasser: RENARD JEAN-PAUL,ZHOU QI,COZZI JEAN
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creator RENARD JEAN-PAUL,ZHOU QI,COZZI JEAN
description Provided is a method for production of reconstituted rat embryos by nuclear transfer including the following steps: (a) removal of a rat ovocyte and maintenance in an appropriate medium; b) insertion of a rat cell nucleus into the recipient rat ovocyte previously maintained in vitro to obtain a reconstituted ovocyte (nuclear transfer); (c) activation of the reconstituted ovocyte obtained in step b) to obtain a reconstituted embryo. The invention further relates to the rats obtained thus, in the foetal or adult state, as well as use thereof for the production of molecules of interest or as study models.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
title Method for cloning in the rat by nuclear transfer
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