Detection method of male sterality and preparation method of its detecting reagent

A method for testing the male sterility includes preparing test reagent for testing enzyme activity, preparing sperm speciman, and enzymatic reaction to test the activity of alpha-glucosidase. A process for preparing said test reagent includes preparing pentacetoxy glucoside, preparing 2-chloro-4-nitrophenyl-alpha-tetraetoxy glucoside, preparing 2-chloro-4-nitrophenyl-alpha-D-glucoside, and mixing the solidified buffering liquid, 2-chloro-4-nitrophenyl-alpha-D-glucoside, and reductive glutathion to obtain powder. 本发明关于一种男性不育症的检测方法及其检测试剂的制备方法。其中检测方法包括：制备含2-氯-4-硝基苯基-α-D-葡萄糖苷的酶活力检测试剂液；制备精浆样品，在测定的条件下进行酶促反应，检测α-葡萄糖苷酶的活性。该检测试剂的制备方法包括：制备五乙酰氧基葡萄糖苷；采用得到的五乙酰氧基葡萄糖苷制备2-氯-4-硝基苯基-α-四乙酰氧基葡萄糖苷；用2-氯-4-硝基苯基-α-四乙酰氧基葡萄糖苷制备2-氯-4-硝基苯基-α-D-葡萄糖苷；和取适量缓冲液固体、2-氯-4-硝基苯基-α-D-葡萄糖苷和还原型谷胱甘肽混合制成粉剂，并确定检测方法，以适应仪器自动化分析。