Method of fast detecting beta2-acceptor excitant in biological sample and its application

The detecting method is the combination of derivation solid phase micro extraction technology and gas chromatography/mass spectroscopy. The matter to be measured is separated from the sample and enriched onto SPME fiber by its adsorbing affinity to the surface coating of the solid phase micro extracting fiber; the fiber is then set in the gas phase of the deriving agent for derivation reaction; and the fiber is finally detected in gas chromatography/mass spectroscopy system. In the selective ionic detection, the detected peak area is compared with contrast curve to calculate the beta2-acceptor excitant density in tested sample. The present invention has the advantages of simple operation, fast analysis, high accuracy, high sensitivity, etc. 本发明提供的快速检测生物样品中β#-[2]-受体兴奋剂的方法，是将衍生化固相微萃取技术与气相色谱/质谱联用，利用待测物对固相微萃取纤维表面涂层的吸附亲和力，使其从样品中分离，并富集于SPME纤维上；再将纤维置于衍生剂的气相顶空中进行衍生化反应；最后于GC/MS系统中检测，采用选择离子检测方式，以峰面积定量，用所得峰面积对照标准曲线计算出待测样品中β#-[2]-受体兴奋剂的浓度；具有操作简单、分析快速、准确性好、灵敏度高、所需有机溶剂少、经济、无毒害、无污染等优点。