Characterisation of gene function using double stranded RNA inhibition

A plant cell comprises a DNA sequence from a plant pest which is critical for the plant pest's survival, growth, proliferation or reproduction. The DNA sequence or a fragment thereof is cloned in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable o...

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Hauptverfasser: G. PLAETINCK, C. PLATTEEUW, K. MORTIER
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creator G. PLAETINCK
C. PLATTEEUW
K. MORTIER
description A plant cell comprises a DNA sequence from a plant pest which is critical for the plant pest's survival, growth, proliferation or reproduction. The DNA sequence or a fragment thereof is cloned in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). The plant pest may be a plant pest that feeds on the plant. Also provided is a plant comprising the plant cell. Also provided is a method of producing a plant resistant to plant pest infestation, which method comprises identifying a DNA sequence from said pest which is critical for its survival, growth, proliferation, or reproduction. The method also comprises cloning the DNA sequence or a fragment thereof in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). The method also comprises introducing said vector into the plant.
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MORTIER</creatorcontrib><description>A plant cell comprises a DNA sequence from a plant pest which is critical for the plant pest's survival, growth, proliferation or reproduction. The DNA sequence or a fragment thereof is cloned in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). The plant pest may be a plant pest that feeds on the plant. Also provided is a plant comprising the plant cell. Also provided is a method of producing a plant resistant to plant pest infestation, which method comprises identifying a DNA sequence from said pest which is critical for its survival, growth, proliferation, or reproduction. The method also comprises cloning the DNA sequence or a fragment thereof in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). 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The method also comprises cloning the DNA sequence or a fragment thereof in a suitable vector in an orientation relative to promoter(s) such that said promoter(s) is capable of initiating transcription of said DNA sequence to dsRNA upon binding of an appropriate transcription factor to said promoter(s). 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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
title Characterisation of gene function using double stranded RNA inhibition
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