Process for preparing recombinant proteins using highly efficient expression vector from accharomyces cerevisiae

This invention concerns a method of producing human granulocyte colony-stimulating factor (hGCSF) and human growth hormone (hGH) from yeast by using recombinant DNA technology. More specifically, this invention relates to a method of producing hGCSF or hGH by using yeast expression vector which cont...

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Hauptverfasser: BAE CHEON-SOON, WOON JAE-WOONG, JANG KI-RYONG
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creator BAE CHEON-SOON
WOON JAE-WOONG
JANG KI-RYONG
description This invention concerns a method of producing human granulocyte colony-stimulating factor (hGCSF) and human growth hormone (hGH) from yeast by using recombinant DNA technology. More specifically, this invention relates to a method of producing hGCSF or hGH by using yeast expression vector which contains: hybrid promoter comprising promoters of two different yeast-derived genes, yeast killer toxinleader peptide, and amino terminus of IL-1β. In addition, this invention relates to a method of producing hGCSF by using expression vector which contains promoter and secretion signal of HSP 150.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Process for preparing recombinant proteins using highly efficient expression vector from accharomyces cerevisiae
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