Detection and digital quantification of multiple targets

The present disclosure provides compositions, methods, and systems for performing highly multiplexed molecular diagnostic assays involving color combination, stimulus response probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in digital assays. Multiplexed detection of targets is achieved in a fast manner with respect to sample zoning and target detection using multiple color channels for detection. Implementation of the method also enables detection with significantly improved signal-to-noise ratio (SNR) values. 本公开提供了用于实施涉及颜色组合、刺激响应探针、串联探针、缀合聚合物探针以及用于增加可以在数字测定中被同时检测的靶的数量的其他机制的高度多重化分子诊断测定的组合物、方法和系统。关于使用多个颜色通道进行检测的样品分区和靶检测，靶的多重化检测以快速方式实现。所述方法的实施还实现了具有显著改善的信噪比(SNR)值的检测。