Kit and method for detecting HBP by eliminating endogenous interference
The invention provides a kit for detecting HBP by eliminating endogenous interference and application of the kit. The invention provides a sample pretreatment method, which is used for eliminating the interference of HBP additionally released by neutrophil in a blood sample on detection in a centrif...
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| creator | GONG QING ZHOU XUYI HONG LONGBIN WANG TAO LIU XIAODONG YU HAIPENG ZHANG YANHUA |
| description | The invention provides a kit for detecting HBP by eliminating endogenous interference and application of the kit. The invention provides a sample pretreatment method, which is used for eliminating the interference of HBP additionally released by neutrophil in a blood sample on detection in a centrifugation process, and specifically comprises the following steps: firstly, adding a rabbit HBP antibody into the blood sample to capture HBP in the sample to form a rabbit HBP antibody-HBP compound, then adding HBP antigen polypeptide to seal redundant rabbit HBP antibodies, and finally, detecting the HBP antibody-HBP compound. And finally, detecting the content of the rabbit HBP antibody-HBP compound, and calculating the concentration of HBP in the sample. The blood sample treated by the pretreatment method has no special requirement on the blood collection tube, the placement time of the pretreated blood sample can be prolonged, and a solution is provided for detecting the HBP on a large scale. Various reagents in |
| format | Patent |
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The invention provides a sample pretreatment method, which is used for eliminating the interference of HBP additionally released by neutrophil in a blood sample on detection in a centrifugation process, and specifically comprises the following steps: firstly, adding a rabbit HBP antibody into the blood sample to capture HBP in the sample to form a rabbit HBP antibody-HBP compound, then adding HBP antigen polypeptide to seal redundant rabbit HBP antibodies, and finally, detecting the HBP antibody-HBP compound. And finally, detecting the content of the rabbit HBP antibody-HBP compound, and calculating the concentration of HBP in the sample. The blood sample treated by the pretreatment method has no special requirement on the blood collection tube, the placement time of the pretreated blood sample can be prolonged, and a solution is provided for detecting the HBP on a large scale. 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The invention provides a sample pretreatment method, which is used for eliminating the interference of HBP additionally released by neutrophil in a blood sample on detection in a centrifugation process, and specifically comprises the following steps: firstly, adding a rabbit HBP antibody into the blood sample to capture HBP in the sample to form a rabbit HBP antibody-HBP compound, then adding HBP antigen polypeptide to seal redundant rabbit HBP antibodies, and finally, detecting the HBP antibody-HBP compound. And finally, detecting the content of the rabbit HBP antibody-HBP compound, and calculating the concentration of HBP in the sample. The blood sample treated by the pretreatment method has no special requirement on the blood collection tube, the placement time of the pretreated blood sample can be prolonged, and a solution is provided for detecting the HBP on a large scale. 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The invention provides a sample pretreatment method, which is used for eliminating the interference of HBP additionally released by neutrophil in a blood sample on detection in a centrifugation process, and specifically comprises the following steps: firstly, adding a rabbit HBP antibody into the blood sample to capture HBP in the sample to form a rabbit HBP antibody-HBP compound, then adding HBP antigen polypeptide to seal redundant rabbit HBP antibodies, and finally, detecting the HBP antibody-HBP compound. And finally, detecting the content of the rabbit HBP antibody-HBP compound, and calculating the concentration of HBP in the sample. The blood sample treated by the pretreatment method has no special requirement on the blood collection tube, the placement time of the pretreated blood sample can be prolonged, and a solution is provided for detecting the HBP on a large scale. Various reagents in</abstract><oa>free_for_read</oa></addata></record> |
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| title | Kit and method for detecting HBP by eliminating endogenous interference |
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