Cellobiose epimerase mutant and application thereof

The invention relates to the technical field of biology, in particular to a cellobiose epimerase mutant and application thereof. According to the cellobiose epimerase mutant and application provided by the embodiment of the invention, the cellobiose epimerase mutant generates mutation which is generated at at least one key site in an amino acid sequence as shown in SEQ ID NO: 51 and is beneficial to improving the catalytic activity of lactulose formed by lactose epimerization; through the mode, the cellobiose epimerase mutant shows excellent catalytic activity for forming lactulose through lactose epimerization, lactose can be efficiently converted into lactulose, and the synthesis efficiency of lactulose can be improved. 本申请涉及生物技术领域，特别是涉及一种纤维二糖差向异构酶突变体及应用。本申请实施例提供的用于纤维二糖差向异构酶突变体及应用，该纤维二糖差向异构酶突变体产生在如SEQ ID NO：51所示氨基酸序列中至少一个关键位点产生有利于提高乳糖差向异构化形成乳果糖催化活性的突变；通过上述方式，该纤维二糖差向异构酶突变体表现出优异的乳糖差向异构化形成乳果糖催化活性，能够高效的将乳糖转换为乳果糖，有利于提高乳果糖合成效率。