Immunological detection method and immunological detection kit

According to the present invention, provided is a method for immunological detection of a trimeric collagen type I N-terminal propeptide in a biological sample using: a first antibody that binds to a first specific moiety of a pro-alpha 1 chain in the trimeric collagen type I N-terminal propeptide, and a second antibody that binds to a second specific moiety of a pro-alpha 1 chain in the trimeric collagen type I N-terminal propeptide; and a second antibody that binds to a second specific moiety of the pro-[alpha] 1 chain in the trimer type I collagen N-terminal propeptide. The immunological detection method is simple to operate, and can specifically (selectively) measure trimers in a biological sample containing a trimer of an alpha chain of PINP and a monomer. 根据本发明，提供一种生物体样品中的三聚体I型胶原N端前肽的免疫学检测方法，其使用：第1抗体，其与所述三聚体I型胶原N端前肽中的pro-α1链的第1特定部分结合；和第2抗体，其与所述三聚体I型胶原N端前肽中的pro-α1链的第2特定部分结合。所述免疫学检测方法操作简便，并且，可以特异性地(选择性地)对包含PINP的α链的三聚体和单体的生物体样品中的三聚体进行测定。