Insertion and deletion molecular marker of rice salt-tolerant gene OsCam1-1, application of insertion and deletion molecular marker, primer pair and identification method

The invention relates to the technical field of gene engineering, in particular to an insertion and deletion molecular marker of a rice salt-tolerant gene OsCam1-1 as well as application, a primer pair and an identification method of the insertion and deletion molecular marker. The physical position...

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Bibliographische Detailangaben
Hauptverfasser: GENG LEIYUE, ZOU TUO, WANG SHUO, YAO YUTAO, DU QI, ZHENG ZHENYU, ZHANG WEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of gene engineering, in particular to an insertion and deletion molecular marker of a rice salt-tolerant gene OsCam1-1 as well as application, a primer pair and an identification method of the insertion and deletion molecular marker. The physical position of the insertion and deletion molecular marker is located at the-1205bp to-1215bp of the promoter genome sequence of the rice salt-tolerant gene OsCam1-1, the basic group is ACATGATTGA, and the basic group is deleted from the salt-tolerant rice variety. By screening the molecular marker closely linked with the salt-tolerant gene, the rice variety with strong salt tolerance can be identified and obtained. The molecular marker disclosed by the invention can be used for identifying and selecting salt-tolerant genotypes in the germination period of rice seeds, so that individuals carrying excellent salt-tolerant allelic variation are obtained, the defects of long required time period and the like of a conventional bre