Method for detecting rice single-cell gene sequence variation and application thereof

The invention provides a method for detecting rice single-cell gene sequence variation and application thereof, and belongs to the technical field of agriculture. The method disclosed by the invention mainly comprises three aspects of sampling, sequencing and biosignal analysis, wherein single rice microspore cells are obtained by sampling. According to the method, the single rice microspore cell is successfully separated, the defects of poor integrity, to-be-improved detection continuity and the like of the existing rice single-cell variation detection technology flow are overcome, and in addition, the authenticity of variation sites is identified and confirmed through a heavy ion mutagenesis technology. The method provided by the invention widens the source range of the rice single-cell mutation material, and provides a new idea and method for creating more diversified breeding materials and performing more efficient mutation breeding. Meanwhile, the microspore cells serve as male gametes, mutation carried