Fermentation method of pseudomonas for producing diastase

The invention belongs to the technical field of strain fermentation, and discloses a fermentation method of pseudomonas for producing diastase. Comprising the steps that the fermentation time is 44.5 h, the fermentation temperature is 22 DEG C, the initial fermentation pH is 6.0, the inoculation amo...

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Bibliographische Detailangaben
Hauptverfasser: CHI XUEMEI, WU JIAMING, CHI NAIYU, YU SHUANG, ZHANG QINGFANG, WANG YAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of strain fermentation, and discloses a fermentation method of pseudomonas for producing diastase. Comprising the steps that the fermentation time is 44.5 h, the fermentation temperature is 22 DEG C, the initial fermentation pH is 6.0, the inoculation amount is 1 vt%, the liquid loading amount is 110 ml/250 mL, and the rotating speed is 191 r/min. The enzyme activity can be effectively improved, and a foundation is laid for converting laboratory research results into large-scale industrial production. 本发明属于菌株发酵技术领域,公开了一种产生淀粉糖化酶假单胞菌发酵方法。包括发酵时间44.5h、发酵温度22℃、发酵起始pH 6.0、接种量1vt%、装液量110ml/250mL,转速191r/min。可有效提高酶活,为将实验室研究结果转化为大规模工业化生产奠定基础。