Method for enriching vesicles with organic complex
The invention belongs to the technical field of biological medicines, and particularly relates to a method for enriching vesicles with organic complexes. The method comprises the following steps: adding a metal salt and an organic complex into an organic solvent, stirring, heating, and drying to obt...
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| creator | WANG BANGMING LI JIAHAO KONG WEIHENG ZHAO YAN |
| description | The invention belongs to the technical field of biological medicines, and particularly relates to a method for enriching vesicles with organic complexes. The method comprises the following steps: adding a metal salt and an organic complex into an organic solvent, stirring, heating, and drying to obtain MOF; dissolving MOF in pure water to obtain an MOF solution; and adding an MOF solution into the bacterial liquid, incubating, centrifuging and redissolving to obtain the vesicles. The method for enriching the bacterial outer vesicles from the biological sample by utilizing the metal MOF is provided for the first time, the problem that the traditional vesicle extraction yield is not high is greatly improved by utilizing the combination of the metal MOF and the vesicles, the quality of the vesicles is improved, the vesicles can be centrifuged at a lower rotating speed, and the requirement on an instrument is reduced. The metal MOF is used for extracting the outer vesicles from the simply centrifuged bacterial li |
| format | Patent |
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The method comprises the following steps: adding a metal salt and an organic complex into an organic solvent, stirring, heating, and drying to obtain MOF; dissolving MOF in pure water to obtain an MOF solution; and adding an MOF solution into the bacterial liquid, incubating, centrifuging and redissolving to obtain the vesicles. The method for enriching the bacterial outer vesicles from the biological sample by utilizing the metal MOF is provided for the first time, the problem that the traditional vesicle extraction yield is not high is greatly improved by utilizing the combination of the metal MOF and the vesicles, the quality of the vesicles is improved, the vesicles can be centrifuged at a lower rotating speed, and the requirement on an instrument is reduced. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for enriching vesicles with organic complex |
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