Preparation and application of monoclonal antibody of anti-CD19 antibody
The invention relates to the field of biological medicine, in particular to preparation and application of a monoclonal antibody of an anti-CD19 antibody. The anti-CD19-ScFv monoclonal antibody B93-7D4 is obtained by immunizing mice through ScFv-CD19 and carrying out mass screening and subcloning, t...
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| creator | HONG JUAN GUO XIANG QIN LEI JIAO LIURONG LIU TONGCAN ZHAO WENXU CHEN XINGYU WANG MEILING |
| description | The invention relates to the field of biological medicine, in particular to preparation and application of a monoclonal antibody of an anti-CD19 antibody. The anti-CD19-ScFv monoclonal antibody B93-7D4 is obtained by immunizing mice through ScFv-CD19 and carrying out mass screening and subcloning, the monoclonal antibody B93-7D4 can be specifically combined with CAR-T ScFv of which the target point is CD19, such as FMC63ScFv and humanized ScFv, the monoclonal antibody B93-7D4 is applied to detection of CD19 CAR positive cells, the detection specificity is high, the detection result is accurate and reliable, the use amount of a detection reagent is small, the detection cost is low, and the monoclonal antibody B93-7D4 is suitable for clinical application. The method has a wide application prospect in the field of immunotherapy, and has important guiding significance for the preparation quality and clinical application of the CAR-T.
本发明涉及生物医药领域，具体涉及抗CD19抗体的单克隆抗体制备及应用。本发明通过ScFv-CD19免疫小鼠，并经过大量筛选及亚克隆，获得了一种抗CD19-ScF |
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本发明涉及生物医药领域，具体涉及抗CD19抗体的单克隆抗体制备及应用。本发明通过ScFv-CD19免疫小鼠，并经过大量筛选及亚克隆，获得了一种抗CD19-ScF</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240913&DB=EPODOC&CC=CN&NR=118638238A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240913&DB=EPODOC&CC=CN&NR=118638238A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HONG JUAN</creatorcontrib><creatorcontrib>GUO XIANG</creatorcontrib><creatorcontrib>QIN LEI</creatorcontrib><creatorcontrib>JIAO LIURONG</creatorcontrib><creatorcontrib>LIU TONGCAN</creatorcontrib><creatorcontrib>ZHAO WENXU</creatorcontrib><creatorcontrib>CHEN XINGYU</creatorcontrib><creatorcontrib>WANG MEILING</creatorcontrib><title>Preparation and application of monoclonal antibody of anti-CD19 antibody</title><description>The invention relates to the field of biological medicine, in particular to preparation and application of a monoclonal antibody of an anti-CD19 antibody. The anti-CD19-ScFv monoclonal antibody B93-7D4 is obtained by immunizing mice through ScFv-CD19 and carrying out mass screening and subcloning, the monoclonal antibody B93-7D4 can be specifically combined with CAR-T ScFv of which the target point is CD19, such as FMC63ScFv and humanized ScFv, the monoclonal antibody B93-7D4 is applied to detection of CD19 CAR positive cells, the detection specificity is high, the detection result is accurate and reliable, the use amount of a detection reagent is small, the detection cost is low, and the monoclonal antibody B93-7D4 is suitable for clinical application. The method has a wide application prospect in the field of immunotherapy, and has important guiding significance for the preparation quality and clinical application of the CAR-T.
本发明涉及生物医药领域，具体涉及抗CD19抗体的单克隆抗体制备及应用。本发明通过ScFv-CD19免疫小鼠，并经过大量筛选及亚克隆，获得了一种抗CD19-ScF</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPAIKEotSCxKLMnMz1NIzEtRSCwoyMlMhvDz0xRy8_Pyk3Py8xJzgLIlmUn5KZUgYRBb19nF0BIuysPAmpaYU5zKC6W5GRTdXEOcPXRTC_LjU4sLEpNT81JL4p39DA0tzIwtjIwtHI2JUQMAgxAz5Q</recordid><startdate>20240913</startdate><enddate>20240913</enddate><creator>HONG JUAN</creator><creator>GUO XIANG</creator><creator>QIN LEI</creator><creator>JIAO LIURONG</creator><creator>LIU TONGCAN</creator><creator>ZHAO WENXU</creator><creator>CHEN XINGYU</creator><creator>WANG MEILING</creator><scope>EVB</scope></search><sort><creationdate>20240913</creationdate><title>Preparation and application of monoclonal antibody of anti-CD19 antibody</title><author>HONG JUAN ; GUO XIANG ; QIN LEI ; JIAO LIURONG ; LIU TONGCAN ; ZHAO WENXU ; CHEN XINGYU ; WANG MEILING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118638238A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HONG JUAN</creatorcontrib><creatorcontrib>GUO XIANG</creatorcontrib><creatorcontrib>QIN LEI</creatorcontrib><creatorcontrib>JIAO LIURONG</creatorcontrib><creatorcontrib>LIU TONGCAN</creatorcontrib><creatorcontrib>ZHAO WENXU</creatorcontrib><creatorcontrib>CHEN XINGYU</creatorcontrib><creatorcontrib>WANG MEILING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HONG JUAN</au><au>GUO XIANG</au><au>QIN LEI</au><au>JIAO LIURONG</au><au>LIU TONGCAN</au><au>ZHAO WENXU</au><au>CHEN XINGYU</au><au>WANG MEILING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation and application of monoclonal antibody of anti-CD19 antibody</title><date>2024-09-13</date><risdate>2024</risdate><abstract>The invention relates to the field of biological medicine, in particular to preparation and application of a monoclonal antibody of an anti-CD19 antibody. The anti-CD19-ScFv monoclonal antibody B93-7D4 is obtained by immunizing mice through ScFv-CD19 and carrying out mass screening and subcloning, the monoclonal antibody B93-7D4 can be specifically combined with CAR-T ScFv of which the target point is CD19, such as FMC63ScFv and humanized ScFv, the monoclonal antibody B93-7D4 is applied to detection of CD19 CAR positive cells, the detection specificity is high, the detection result is accurate and reliable, the use amount of a detection reagent is small, the detection cost is low, and the monoclonal antibody B93-7D4 is suitable for clinical application. The method has a wide application prospect in the field of immunotherapy, and has important guiding significance for the preparation quality and clinical application of the CAR-T.
本发明涉及生物医药领域，具体涉及抗CD19抗体的单克隆抗体制备及应用。本发明通过ScFv-CD19免疫小鼠，并经过大量筛选及亚克隆，获得了一种抗CD19-ScF</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Preparation and application of monoclonal antibody of anti-CD19 antibody |
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