Recombinant humanized I-type collagen as well as expression vector and genetically engineered bacterium thereof
The invention discloses a recombinant humanized I-type collagen as well as an expression vector and a genetically engineered bacterium thereof. According to the invention, functional domains combined with cell integrin and fibronectin in human I-type collagen are screened and combined, or carboxyl t...
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| creator | TAN CHENGLONG JI LE ZHAO JIANFENG ZHOU JIE ZHU YILI |
| description | The invention discloses a recombinant humanized I-type collagen as well as an expression vector and a genetically engineered bacterium thereof. According to the invention, functional domains combined with cell integrin and fibronectin in human I-type collagen are screened and combined, or carboxyl terminal peptides form recombinant humanized I-type collagen JYC103 and JYC104, the recombinant humanized I-type collagen JYC103 and JYC104 are amplified and cloned into a pPIC9K vector after codon optimization, the recombinant humanized I-type collagen JYC104 is converted into pichia pastoris cells after enzyme digestion linearization, and the recombinant humanized I-type collagen JYC103 and JYC104 is obtained. And high-copy recombinants are screened through G418 resistance gradient, so that the pichia pastoris genetically engineered bacteria capable of expressing the recombinant humanized I-type collagen JYC103 and JYC104 are obtained. The recombinant humanized I-type collagen JYC103 and JYC104 have excellent func |
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According to the invention, functional domains combined with cell integrin and fibronectin in human I-type collagen are screened and combined, or carboxyl terminal peptides form recombinant humanized I-type collagen JYC103 and JYC104, the recombinant humanized I-type collagen JYC103 and JYC104 are amplified and cloned into a pPIC9K vector after codon optimization, the recombinant humanized I-type collagen JYC104 is converted into pichia pastoris cells after enzyme digestion linearization, and the recombinant humanized I-type collagen JYC103 and JYC104 is obtained. And high-copy recombinants are screened through G418 resistance gradient, so that the pichia pastoris genetically engineered bacteria capable of expressing the recombinant humanized I-type collagen JYC103 and JYC104 are obtained. 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According to the invention, functional domains combined with cell integrin and fibronectin in human I-type collagen are screened and combined, or carboxyl terminal peptides form recombinant humanized I-type collagen JYC103 and JYC104, the recombinant humanized I-type collagen JYC103 and JYC104 are amplified and cloned into a pPIC9K vector after codon optimization, the recombinant humanized I-type collagen JYC104 is converted into pichia pastoris cells after enzyme digestion linearization, and the recombinant humanized I-type collagen JYC103 and JYC104 is obtained. And high-copy recombinants are screened through G418 resistance gradient, so that the pichia pastoris genetically engineered bacteria capable of expressing the recombinant humanized I-type collagen JYC103 and JYC104 are obtained. 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According to the invention, functional domains combined with cell integrin and fibronectin in human I-type collagen are screened and combined, or carboxyl terminal peptides form recombinant humanized I-type collagen JYC103 and JYC104, the recombinant humanized I-type collagen JYC103 and JYC104 are amplified and cloned into a pPIC9K vector after codon optimization, the recombinant humanized I-type collagen JYC104 is converted into pichia pastoris cells after enzyme digestion linearization, and the recombinant humanized I-type collagen JYC103 and JYC104 is obtained. And high-copy recombinants are screened through G418 resistance gradient, so that the pichia pastoris genetically engineered bacteria capable of expressing the recombinant humanized I-type collagen JYC103 and JYC104 are obtained. The recombinant humanized I-type collagen JYC103 and JYC104 have excellent func</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS VINEGAR WINE |
| title | Recombinant humanized I-type collagen as well as expression vector and genetically engineered bacterium thereof |
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