Fluorescent PCR kit for detecting KRAS gene mutation of circulating tumor cells of colorectal cancer and detection method thereof

The invention relates to the field of genetic engineering and molecular biology, and discloses a fluorescent PCR kit for detecting KRAS gene mutation of circulating tumor cells of colorectal cancer and a detection method thereof, the fluorescent PCR kit comprises a KRAS specific primer and a KRAS probe which are required for detecting the KRAS gene mutation, and a reference gene primer and a reference gene probe; the KRAS specific primer is used for amplifying a target area in a single circulating tumor cell sample to be detected to obtain an amplification product; the specific primer comprises a first upstream primer and a first downstream primer; the KRAS probe is used for carrying out hydrolysis reaction in the generation process of the amplification product and releasing a fluorescence signal; the internal reference gene primer is used for amplifying a housekeeping gene in the to-be-detected CTC to obtain an amplification product, and the internal reference gene primer comprises an internal reference upst