LAMP primer group and kit for detecting novel coronavirus nucleic acid based on LFIA and LAMP technologies

The invention relates to the field of molecular biology, and discloses an LAMP primer group and a kit for detecting novel coronavirus nucleic acid based on LFIA and LAMP technologies, and the primer group comprises an LAMP primer group aiming at an SARS-CoV-2 specific gene N gene and an LAMP primer...

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Hauptverfasser: ZHOU XIANGMIN, DIAO QIZHI, ZHU GUOQIANG, YANG FANGYU, GUO CHAOZHONG, YANG YUKUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the field of molecular biology, and discloses an LAMP primer group and a kit for detecting novel coronavirus nucleic acid based on LFIA and LAMP technologies, and the primer group comprises an LAMP primer group aiming at an SARS-CoV-2 specific gene N gene and an LAMP primer group aiming at an SARS-CoV-2 specific gene Orf1ab gene. According to the method, the usability and timeliness of detection can be greatly improved, the detection period is greatly shortened, meanwhile, the dependence on high-end experimental facilities is reduced, and accurate screening can be achieved when an infected person does not have obvious symptoms or the virus load is still low. 本发明涉及分子生物学领域,公开了一种基于LFIA及LAMP技术检测新型冠状病毒核酸的LAMP引物组和试剂盒,引物组包括针对SARS-CoV-2特异性基因N基因的LAMP引物组和针对SARS-CoV-2特异性基因Orf1ab基因的LAMP引物组。本发明能够极大地提升了检测的易用性和时效性,大幅缩减了检测周期,同时降低了对高端实验设施的依赖,能够在感染者尚未出现明显症状或病毒载量尚低时,就实现精准筛查。