High performance liquid chromatography detection method of vitamin H intermediate chiral lactone

The invention provides a high performance liquid chromatography detection method of vitamin H intermediate chiral lactone, which comprises the following steps: 1) carrying out derivation reaction on right lactone as shown in a formula 1, left lactone as shown in a formula 2 and chiral amine to obtain different chiral amide derivatives; and 2) carrying out separation detection on different chiral amide derivatives by high performance liquid chromatography. The lactone enantiomer derivative disclosed by the invention has a relatively good separation effect. 本发明提供了一种维生素H中间体手性内酯的高效液相色谱检测方法，所述高效液相色谱检测方法包括如下步骤：1)将如式1所示的右内酯和如式2所示的左内酯与手性胺进行衍生法反应，得到不同手性酰胺衍生物；2)将不同手性酰胺衍生物通过高效液相色谱法进行分离检测。本发明的内酯对映体衍生物获得了较好的分离效果。