Rapid detection method for 18bp indel polymorphism of bovine CLCF1 gene and application of rapid detection method

The invention discloses a rapid detection method for 18bp indel polymorphism of a bovine CLCF1 gene and application of the rapid detection method, and belongs to the field of molecular genetics. The detection method comprises the following steps: designing upstream and downstream primers of the site to be expressed by P according to the characteristic that 18bp insertion exists in a second intron region of the CLCF1 gene. Jugular vein blood collection and genome DNA extraction are carried out on detected different varieties of cattle, PCR amplification is carried out through a primer pair P, finally, the 18bp indel polymorphism of the gene is detected through agarose gel electrophoresis, when 18bp insertion exists, the gene is the II genotype, when 18bp deletion exists, the gene is the DD genotype, and when the site is a heterozygous individual, the gene is the ID genotype. Meanwhile, genotype distribution and allele frequency of different varieties of cattle are further analyzed, correlation analysis is carr