Fluorescent PCR (polymerase chain reaction) kit for jointly detecting multiple mutation sites of EGFR (epidermal growth factor receptor) genes in lung cancer circulating tumor cells and detection method of fluorescent PCR kit

The invention relates to the field of gene engineering and molecular biology, and discloses a fluorescent PCR kit for combined detection of EGFR gene multiple mutation sites in lung cancer circulating tumor cells and a detection method thereof, the fluorescent PCR kit comprises EGFR specific primers and EGFR probes required for detection of EGFR gene mutation; a reference gene primer and a reference gene probe; the EGFR specific primer is used for amplifying a target area in a single circulating tumor cell sample to be detected to obtain an amplification product; the EGFR probe is used for carrying out hydrolysis reaction in the generation process of the amplification product and releasing a fluorescence signal; the internal reference gene primer is used for amplifying a housekeeping gene in the CTC to be detected to obtain an amplification product, and comprises an internal reference upstream primer and an internal reference downstream primer; and the reference gene probe is used for carrying out hydrolysis