Molecular method for identifying muscle fibers by using skeletal muscle exosome miRNA

The invention discloses a molecular method for performing muscle fiber identification by using skeletal muscle exosome miRNA, and belongs to the technical field of molecular biology. S1, miRNA transcriptome sequencing: performing miRNA sequencing analysis on oxidized skeletal muscle (lumbar major muscle) and glycolytic skeletal muscle (longissimus dorsi muscle) and exosomes thereof of Hechuan pigs; s2, analyzing differential miRNAs of oxidized and glycolytic skeletal muscle tissues and exosomes; s3, studying the promotion of the miR-4331-3p on the proliferation of myoblasts; and S4, researching the inhibition of the miR-4331-3p on myoblast differentiation and muscle fiber formation. Sequencing analysis is carried out on the skeletal muscle and exosome miRNA of the skeletal muscle, it is found that miRNAs in skeletal muscle exosomes of different metabolism types have unique expression characteristics and show different modes from skeletal muscle tissue from the skeletal muscle exosomes, it is indicated that co