METHOD FOR DEVELOPING CELL LINES FOR PRODUCING VIRUS IN SUSPENSION CELL CULTURE

A method of generating a cell line for producing a virus in suspension cell culture. The method comprises: generating a plurality of cell clones growing in an adherent cell culture; identifying a group of cell clones with high transfection efficiency; generating a plurality of cell clones growing in the suspension cell culture; and identifying a cell clone suitable for producing the virus in the suspension cell culture. According to the method disclosed by the invention, the domestication time can be shortened from at least one month to 7-14 days, and the amount of culture medium used during the domestication process can be greatly reduced, so that the production cost is reduced. 一种生成细胞系的方法，所述细胞系用于在悬浮细胞培养中产生病毒。所述方法包括：生成在贴壁细胞培养中生长的多个细胞克隆；鉴定具有高转染效率的一组细胞克隆；生成在悬浮细胞培养中生长的多个细胞克隆；以及鉴定适合于在悬浮细胞培养中产生病毒的细胞克隆。本文所公开的方法中的所述方法可以将驯化时间从至少1个月缩短到7-14天，并且还可以大大减少驯化过程期间使用的培养基的量，从而降低生产成本。