Method for rapidly extracting plant cell nucleus
The invention is applicable to the technical field of cell nucleus extraction, and provides a method for rapidly extracting plant cell nucleuses, which comprises the following steps: taking 1g of plant tissues; the method comprises the following steps: grinding plant tissues into powder in liquid ni...
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| creator | CHEN YANYU LIU CAOZHI |
| description | The invention is applicable to the technical field of cell nucleus extraction, and provides a method for rapidly extracting plant cell nucleuses, which comprises the following steps: taking 1g of plant tissues; the method comprises the following steps: grinding plant tissues into powder in liquid nitrogen, and transferring the powder into a pre-cooled 50ml tube containing 30ml of cell nucleus extracting solution; reversing the tube and uniformly mixing, so that the powder is completely dissolved in the cell nucleus extracting solution; carrying out double-layer Mircloth filtering to obtain a clean 50ml centrifugal tube; and centrifuging for 5 minutes, discarding the supernatant, adding 1ml of cell nucleus preserving fluid, and resuspending to obtain a cell nucleus solution. The components of the plant cell nucleus extraction reagent are optimized, and the plant cell nucleus extraction reagent is simple in components, easy to prepare, universal and suitable for cell nucleus extraction of various plants, and th |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for rapidly extracting plant cell nucleus |
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