Pichia pastoris strain for producing ergothioneine as well as construction method and application of pichia pastoris strain

The invention relates to the technical field of synthesis of a cosmetic raw material ergothioneine, and particularly discloses a pichia pastoris strain for producing ergothioneine as well as a construction method and application of the pichia pastoris strain. The pichia pastoris strain is used for simultaneously overexpressing a Tregt1 gene and a Tregt2 gene, and the amino acid sequence of the Tregt1 gene is as shown in SEQ ID NO: 1; the amino acid sequence of the Tregt2 gene is as shown in SEQ ID NO: 2. According to the invention, the ergothioneine synthesis pathway genes Tregt1 and Tregt2 are expressed in the pichia pastoris, and meanwhile, the efficient synthesis of the ergothioneine can be realized in combination with supplementary feeding, so that a new pathway is provided for the efficient synthesis of the ergothioneine. 本发明涉及化妆品原料麦角硫因合成技术领域，具体公开了一种生产麦角硫因的毕赤酵母菌株及其构建方法和应用。所述毕赤酵母菌株同时过表达Tregt1基因、Tregt2基因，所述Tregt1基因的氨基酸序列如SEQ ID NO:1所示；所述Tregt2基因的氨基酸序列如SEQ ID NO:2所示。本发明通过在毕赤酵母中表达麦角硫因合成途径基因Tregt1和Tregt2，同时结合