Directional enrichment method of cucurbitacine containing alpha and beta unsaturated groups in muskmelon pedicel

The invention belongs to the field of extraction and separation of natural products, and particularly relates to a directional enrichment method of cucurbitacine containing alpha and beta unsaturated groups in muskmelon pedicels. A muskmelon pedicel raw material is crushed, is subjected to reflux extraction with 95% ethanol, and is subjected to vacuum concentration to obtain a total extract; reacting cysteine with the extract, and then adding ethyl acetate and acid water for extraction; at the moment, the cucurbitacine containing alpha and beta unsaturated groups is dissolved in the acid water layer due to addition of cysteine. And finally, alkalizing the acid water by using sodium carbonate, adding iodoacetic acid to promote the dissociation of an addition product, and extracting the dissociated cucurbitacine containing alpha and beta unsaturated groups by using ethyl acetate, thereby achieving the purpose of directional enrichment. The method can effectively reduce the workload of extracting the active natu