Method for extracting and purifying animal digestive tract mucoprotein
The invention relates to a method for extracting and purifying mucoprotein, in particular to a method for extracting and purifying animal digestive tract mucoprotein. The problem that an existing animal digestive tract mucoprotein extraction and purification method cannot achieve the optimal effect...
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| creator | PAN LI ZHAO YUAN BAO NAN XIA JIAJIA FAN XIAPU XU CHENGYU HATA TAKANOBU E TIANJIAO WU ZHENLONG |
| description | The invention relates to a method for extracting and purifying mucoprotein, in particular to a method for extracting and purifying animal digestive tract mucoprotein. The problem that an existing animal digestive tract mucoprotein extraction and purification method cannot achieve the optimal effect is solved. The method comprises the following steps: preparing a solution, obtaining a mucus sample, extracting and preparing a mucus crude extract, and extracting and purifying mucoprotein. The incubation time of the cysteine protease inhibitor is prolonged, so that under the condition of reducing the concentration gradient of the cysteine protease inhibitor, the freshness and activity of extracted protein are guaranteed as much as possible, and the effect of being consistent with the protein extraction concentration is achieved. According to the invention, the centrifugal rotating speed is reduced, so that the extraction difficulty is reduced in the aspects of instrument selection and operability. According to th |
| format | Patent |
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The problem that an existing animal digestive tract mucoprotein extraction and purification method cannot achieve the optimal effect is solved. The method comprises the following steps: preparing a solution, obtaining a mucus sample, extracting and preparing a mucus crude extract, and extracting and purifying mucoprotein. The incubation time of the cysteine protease inhibitor is prolonged, so that under the condition of reducing the concentration gradient of the cysteine protease inhibitor, the freshness and activity of extracted protein are guaranteed as much as possible, and the effect of being consistent with the protein extraction concentration is achieved. According to the invention, the centrifugal rotating speed is reduced, so that the extraction difficulty is reduced in the aspects of instrument selection and operability. 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The problem that an existing animal digestive tract mucoprotein extraction and purification method cannot achieve the optimal effect is solved. The method comprises the following steps: preparing a solution, obtaining a mucus sample, extracting and preparing a mucus crude extract, and extracting and purifying mucoprotein. The incubation time of the cysteine protease inhibitor is prolonged, so that under the condition of reducing the concentration gradient of the cysteine protease inhibitor, the freshness and activity of extracted protein are guaranteed as much as possible, and the effect of being consistent with the protein extraction concentration is achieved. According to the invention, the centrifugal rotating speed is reduced, so that the extraction difficulty is reduced in the aspects of instrument selection and operability. 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The problem that an existing animal digestive tract mucoprotein extraction and purification method cannot achieve the optimal effect is solved. The method comprises the following steps: preparing a solution, obtaining a mucus sample, extracting and preparing a mucus crude extract, and extracting and purifying mucoprotein. The incubation time of the cysteine protease inhibitor is prolonged, so that under the condition of reducing the concentration gradient of the cysteine protease inhibitor, the freshness and activity of extracted protein are guaranteed as much as possible, and the effect of being consistent with the protein extraction concentration is achieved. According to the invention, the centrifugal rotating speed is reduced, so that the extraction difficulty is reduced in the aspects of instrument selection and operability. According to th</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES |
| title | Method for extracting and purifying animal digestive tract mucoprotein |
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