Construction method of tumor intramembrane ossification bone invasion animal model

The invention relates to the technical field of animal model modeling. The invention provides a construction method of a tumor intramembrane ossification bone invasion animal model, which comprises the following steps: (1) mixing a single-cell tumor suspension with matrigel to prepare a tumor suspension; and (2) injecting the tumor suspension prepared in the step (1) into a position 0.1-0.3 cm below an adventitia of a skull center seam of an animal. According to the technical scheme of the invention, the modeling of the bone invasion animal with the ossified bone in the tumor membrane is carried out, and the modeling success rate and the modeling uniformity are obviously improved. The invention provides a new basis and thought for clinical diagnosis and treatment of tumor invasion intramembrane ossified bone. 本发明涉及动物模型建模技术领域。本发明提供了一种肿瘤膜内骨化骨骨侵袭动物模型的构建方法，包括：(1)将单细胞肿瘤悬液与基质胶混合，制备肿瘤悬液；(2)将步骤(1)制得的肿瘤悬液注射入动物的颅中缝骨外膜下0.1-0.3cm处。通过本发明技术方案进行肿瘤膜内骨化骨骨侵袭动物造模，明显提高了造模成功率以及造模的均一性。这为肿瘤侵袭膜内骨化骨的临床诊疗提供了新依据和思路。