High-throughput gene sequencing method and device

The invention is applicable to the technical field of sequencing, and provides a high-throughput gene sequencing method and device.The high-throughput gene sequencing method comprises the steps that Barcode amplification treatment is conducted on nucleic acid samples to be sequenced, and all nucleic acid samples with Barcodes are obtained; performing sample mixing and purification treatment on the nucleic acid samples with Barcodes to obtain purified nucleic acid samples; performing FFPE repair and terminal repair treatment on the purified nucleic acid sample to obtain a repaired nucleic acid sample; connecting the repaired nucleic acid sample with a sequencing joint to obtain a DNA library; and performing sequencing analysis on the DNA library by using the nanopore sequencing chip and a sequencer to obtain a sequencing result. According to the sequencing method for multi-sample mixed library building sequencing, high-throughput sequencing is achieved, meanwhile, the single-sample sequencing cost is reduced,