Extraction kit of circulating nucleic acid and extraction method of circulating nucleic acid
The invention relates to the technical field of nucleic acid extraction, and particularly discloses a circulating nucleic acid extraction kit and a circulating nucleic acid extraction method. The circulating nucleic acid extraction kit provided by the invention comprises a lysis enhancer, protease K...
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| creator | LIU YUFANG ZOU AILAN HOU LINGYAN GUAN XUANLI SUN KEFEI LI XIAOCHEN |
| description | The invention relates to the technical field of nucleic acid extraction, and particularly discloses a circulating nucleic acid extraction kit and a circulating nucleic acid extraction method. The circulating nucleic acid extraction kit provided by the invention comprises a lysis enhancer, protease K, a lysis binding solution, a magnetic bead suspension, a deproteinization solution, a rinsing solution and an eluent, the cracking binding solution and the deproteinization solution respectively contain 10-30% (v/v) of alcoholic solution, and the alcoholic solution is selected from one or more of PEG (polyethylene glycol) 400, PEG 600, PEG 800, PEG 1000, PEG 2000, ethylene glycol, 1, 2-propylene glycol, 1, 4-butanediol, glycerol and pentaerythritol. The extraction kit for circulating nucleic acid provided by the invention can enable a biological sample to fully release nucleic acid crosslinked by protein, can effectively remove large-fragment nucleic acid such as genome, and significantly improves the extraction y |
| format | Patent |
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The circulating nucleic acid extraction kit provided by the invention comprises a lysis enhancer, protease K, a lysis binding solution, a magnetic bead suspension, a deproteinization solution, a rinsing solution and an eluent, the cracking binding solution and the deproteinization solution respectively contain 10-30% (v/v) of alcoholic solution, and the alcoholic solution is selected from one or more of PEG (polyethylene glycol) 400, PEG 600, PEG 800, PEG 1000, PEG 2000, ethylene glycol, 1, 2-propylene glycol, 1, 4-butanediol, glycerol and pentaerythritol. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Extraction kit of circulating nucleic acid and extraction method of circulating nucleic acid |
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