Method for inducing axillary buds of current-year stems of phoebe zhennan tree to germinate and cultivating cluster buds

The invention discloses a method for inducing germination of axillary buds of current-year stems of a big phoebe zhennan tree and cultivating cluster buds, and relates to the technical field of phoebe zhennan cultivation. The invention provides a method for inducing axillary buds of current-year ste...

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Hauptverfasser: LIU LIANGMENG, CHEN PING, LIU XIN, LI JIAMAN, GUO HONGYING, GUO MINJIA, CHEN ZHI, HUANG ZHEN, WANG LIHUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for inducing germination of axillary buds of current-year stems of a big phoebe zhennan tree and cultivating cluster buds, and relates to the technical field of phoebe zhennan cultivation. The invention provides a method for inducing axillary buds of current-year stems of phoebe zhennan trees to germinate and culturing cluster buds. The method comprises the following steps: disinfecting the current-year stems of canopies of phoebe zhennan trees, and then pre-culturing to obtain explants; transferring the explant into a primary culture medium for culturing until axillary buds germinate, transferring the axillary buds into a subculture medium for inducing cluster buds for culturing until new buds grow, culturing after recovering illumination, and finally cutting into single buds for subsequent culture. In the induction process, the formulas of the primary culture medium and the subculture medium are adjusted aiming at the Machilus zhennan with the tree age of 10 years to hundred