Production method of polyglutamic acid independent of glutamic acid
The invention relates to the field of microorganisms, and discloses a glutamic acid-independent polyglutamic acid production method which comprises the following steps: transferring a strain into a liquid seed culture medium, and carrying out shake culture for 16-20 hours until the medium logarithmi...
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| creator | TAN PENGZHI YANG QI JIANG CHAOMING HUANG BINLIANG LAN CHENG DENG GUIPING WU HUADE JIANG CHENGJIAN LU FUHAI |
| description | The invention relates to the field of microorganisms, and discloses a glutamic acid-independent polyglutamic acid production method which comprises the following steps: transferring a strain into a liquid seed culture medium, and carrying out shake culture for 16-20 hours until the medium logarithmic growth stage; and inoculating the cultured seeds into a fermentation culture medium according to a volume ratio of 5-10%, fermenting for 24-48 hours, and detecting the content of polyglutamic acid. In the traditional production method, the preparation of polyglutamic acid depends on glutamic acid as a raw material. However, the strain adopted by the invention can synthesize polyglutamic acid without glutamic acid, so that the supply cost of glutamic acid is saved, the production cost is reduced, another beneficial thought is provided for industrial production of polyglutamic acid, meanwhile, by optimizing culture conditions and a culture medium, the yield of polyglutamic acid can be increased, and the method is s |
| format | Patent |
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In the traditional production method, the preparation of polyglutamic acid depends on glutamic acid as a raw material. However, the strain adopted by the invention can synthesize polyglutamic acid without glutamic acid, so that the supply cost of glutamic acid is saved, the production cost is reduced, another beneficial thought is provided for industrial production of polyglutamic acid, meanwhile, by optimizing culture conditions and a culture medium, the yield of polyglutamic acid can be increased, and the method is s</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240528&DB=EPODOC&CC=CN&NR=118086418A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240528&DB=EPODOC&CC=CN&NR=118086418A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TAN PENGZHI</creatorcontrib><creatorcontrib>YANG QI</creatorcontrib><creatorcontrib>JIANG CHAOMING</creatorcontrib><creatorcontrib>HUANG BINLIANG</creatorcontrib><creatorcontrib>LAN CHENG</creatorcontrib><creatorcontrib>DENG GUIPING</creatorcontrib><creatorcontrib>WU HUADE</creatorcontrib><creatorcontrib>JIANG CHENGJIAN</creatorcontrib><creatorcontrib>LU FUHAI</creatorcontrib><title>Production method of polyglutamic acid independent of glutamic acid</title><description>The invention relates to the field of microorganisms, and discloses a glutamic acid-independent polyglutamic acid production method which comprises the following steps: transferring a strain into a liquid seed culture medium, and carrying out shake culture for 16-20 hours until the medium logarithmic growth stage; and inoculating the cultured seeds into a fermentation culture medium according to a volume ratio of 5-10%, fermenting for 24-48 hours, and detecting the content of polyglutamic acid. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Production method of polyglutamic acid independent of glutamic acid |
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