Preparation method of additive factor for enhancing hybridoma fusion

The invention relates to the technical field of hybridoma fusion, and discloses a preparation method of an additive factor for enhancing hybridoma fusion, which comprises the following steps: S1, preparing a polyethylene glycol solution; s2, preparing a cell survival and growth factor solution; s3, preparing an antioxidant solution; s4, preparing a polysaccharide substance solution; s5, preparing a cell apoptosis inhibitor solution; s6, mixing the solutions: mixing the solutions with the same mass part; s7, filtering the solution: filtering the mixed solution by using a sterile filter; the additive factor is prepared by mixing a polyethylene glycol solution, a cell survival and growth factor solution, an antioxidant solution, a polysaccharide substance solution and a cell apoptosis inhibitor solution. The solutions cooperate with each other to enhance the fusion of the hybridoma in different modes, so that the fusion of the hybridoma is enhanced from multiple angles, and the effect is far better than that of