Method for preparing sterile mouse based on non-surgical embryo transplantation technology

The invention discloses a method for preparing a sterile mouse based on a non-surgical embryo transplantation technology, and belongs to the technical field of biology. The invention discloses a method for preparing a sterile mouse based on a non-surgical embryo transplantation technology, which comprises the following steps of: conveying mature embryos (blastocyst and mulberry embryo) obtained by in-vitro fertilization and culture into the uterus of a sterile pseudopregnant female mouse by using the non-surgical embryo transplantation technology; sterile mice are obtained through the processes of female mouse pregnancy and farrowing, weaned newborn mouse microorganism quality inspection qualification and the like in sequence. The method is high in operability and small in pollution risk, the experimental animal welfare principle is fully considered, and a new way for obtaining sterile mice is developed. 本发明公开了一种基于非手术胚胎移植技术制备无菌小鼠的方法，属于生物技术领域。本发明公开的一种基于非手术胚胎移植技术制备无菌小鼠的方法，通过将体外受精与培养获得的成熟胚胎(囊胚与桑椹胚)，利用非手术胚胎移植技术输送