Tetrahydropyrimidine gene cluster derived from halophila bacillus, mutant and application
The invention discloses an ectoine gene cluster derived from halophila bacillus, a mutant and application, and belongs to the technical field of synthetic biology. The nucleotide sequence of the ectoine gene cluster is shown as SEQ ID NO.7, and the ectoine gene cluster comprises the following three...
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| creator | ZHANG JIE ZHENG DEQIANG LI HAIJUN XU BO WANG QINGBO WANG CHAO HU HONGTAO ZHANG YINGHUA LI ZHEN'AI ZHANG XIN |
| description | The invention discloses an ectoine gene cluster derived from halophila bacillus, a mutant and application, and belongs to the technical field of synthetic biology. The nucleotide sequence of the ectoine gene cluster is shown as SEQ ID NO.7, and the ectoine gene cluster comprises the following three genes: an ectA gene with the nucleotide sequence shown as SEQ ID NO.1, an ectB gene with the nucleotide sequence shown as SEQ ID NO.2 and an ectC gene with the nucleotide sequence shown as SEQ ID NO.3, and the gene cluster is subjected to design mutation optimization to obtain a mutant with the nucleotide sequence shown as SEQ ID NO.8. According to the recombinant strain constructed by using the ectoine gene cluster and the mutant thereof, the synthesis efficiency of ectoine can be improved, the fermentation content of ectoine can be increased, the production cost of ectoine can be reduced, and popularization and application of ectoine are facilitated.
本发明公开了一种喜盐芽孢杆菌来源的四氢嘧啶基因簇、突变体及应用，属于合成生物技术领域。该四氢嘧啶基因簇核酸序列如SEQ ID |
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本发明公开了一种喜盐芽孢杆菌来源的四氢嘧啶基因簇、突变体及应用，属于合成生物技术领域。该四氢嘧啶基因簇核酸序列如SEQ ID </description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240510&DB=EPODOC&CC=CN&NR=118006645A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240510&DB=EPODOC&CC=CN&NR=118006645A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG JIE</creatorcontrib><creatorcontrib>ZHENG DEQIANG</creatorcontrib><creatorcontrib>LI HAIJUN</creatorcontrib><creatorcontrib>XU BO</creatorcontrib><creatorcontrib>WANG QINGBO</creatorcontrib><creatorcontrib>WANG CHAO</creatorcontrib><creatorcontrib>HU HONGTAO</creatorcontrib><creatorcontrib>ZHANG YINGHUA</creatorcontrib><creatorcontrib>LI ZHEN'AI</creatorcontrib><creatorcontrib>ZHANG XIN</creatorcontrib><title>Tetrahydropyrimidine gene cluster derived from halophila bacillus, mutant and application</title><description>The invention discloses an ectoine gene cluster derived from halophila bacillus, a mutant and application, and belongs to the technical field of synthetic biology. The nucleotide sequence of the ectoine gene cluster is shown as SEQ ID NO.7, and the ectoine gene cluster comprises the following three genes: an ectA gene with the nucleotide sequence shown as SEQ ID NO.1, an ectB gene with the nucleotide sequence shown as SEQ ID NO.2 and an ectC gene with the nucleotide sequence shown as SEQ ID NO.3, and the gene cluster is subjected to design mutation optimization to obtain a mutant with the nucleotide sequence shown as SEQ ID NO.8. According to the recombinant strain constructed by using the ectoine gene cluster and the mutant thereof, the synthesis efficiency of ectoine can be improved, the fermentation content of ectoine can be increased, the production cost of ectoine can be reduced, and popularization and application of ectoine are facilitated.
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本发明公开了一种喜盐芽孢杆菌来源的四氢嘧啶基因簇、突变体及应用，属于合成生物技术领域。该四氢嘧啶基因簇核酸序列如SEQ ID </abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Tetrahydropyrimidine gene cluster derived from halophila bacillus, mutant and application |
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