Lentiviral vector and application thereof

The invention relates to the technical field of biology, in particular to a lentiviral vector and application thereof. A protease substrate sequence of a cytoplasm tail region of a chimeric envelope is optimally designed to obtain a series of novel chimeric envelope structures, the obtained envelope structures are used for pseudotyping a lentiviral vector carrying a CAR gene, and compared with reported chimeric envelope BaEVTR or BaEVRless, the chimeric envelope has the advantages that the efficiency is high, and the cost is low. The lentiviral vector with the pseudotyped envelope structure has higher virus titer, the toxicity to packaging cells is not obviously increased, and even the toxicity is weak. The lentiviral vector provided by the invention has higher transduction efficiency on NK, resting T, resting B or mononuclear cells, gamma delta T and HSC. 本发明涉及生物技术领域，具体涉及慢病毒载体及其应用。本发明对嵌合包膜的胞质尾区的蛋白酶底物序列进行了优化设计，得到一系列新型嵌合包膜结构，所得包膜结构用来假型化携带CAR基因的慢病毒载体，相比已报道的嵌合型包膜BaEVTR或BaEVRless，本发明的包膜结构假型化的慢病毒载体具有更高的病毒滴度，并且对包装细